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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
11/02/2009 |
Data da última atualização: |
25/11/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOSA-GÓMEZ, D. R.; BINNECK, E.; MARIN, S. R. R. |
Afiliação: |
Daniel Ricardo Sosa Gómez, CNPSo; Eliseu Binneck, CNPSo; Silvana Regina Rockenbach Marin, CNPSo. |
Título: |
New PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 41.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS, 9., 2008, Warwick. Abstracts... Warwick: Society Invertebrate Pathology, 2008 |
Idioma: |
Inglês |
Conteúdo: |
The variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi ha precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possible with universal primers. Thus, to obtain sequences of the IGS region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or E04, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPO amplification and present in the combination IGS-10 mer amplification, these products were gel-purified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 681 to 1,025 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for Nomuraea rileyi. |
Thesagro: |
Entomologia; Nomuraea Rileyi. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01951naa a2200169 a 4500 001 1471519 005 2009-11-25 008 2008 bl --- 0-- u #d 100 1 $aSOSA-GÓMEZ, D. R. 245 $aNew PCR method to study the intergenic spacer (IGS) region of Nomuraea rileyi (Farlow) Samson. 260 $c2008 520 $aThe variability in the IGS region of the rDNA gene complex has proven useful in the development of PCR primers for strain identification. However, the lack of information on this region in Nomuraea rileyi ha precluded the development of specific primers for PCR amplification. Also, due to the high variability in this region, amplification is not possible with universal primers. Thus, to obtain sequences of the IGS region a new approach was used, a combination of IGS primer with 10-mer oligonucleotides (OD11 or E04, Operon Technologies) were used. The amplification products were visualized by agarose gel electrophoresis. DNA bands were selected for comparison between IGS-10 mer primer product amplification and DNA bands produced by 10-mer amplification. When DNA products were absent in the RAPO amplification and present in the combination IGS-10 mer amplification, these products were gel-purified, ligated into the plasmid vector TOPO TA (lnvitrogen), and amplified in DH10 B Escherichia coli cells. The DNA obtained from positive clones were subjected to Sanger sequencing. The obtained sequences ranged from 681 to 1,025 bases. Comparisons among isolates obtained from the same geographic region suggest that this technique could be used to develop strain specific primers for Nomuraea rileyi. 650 $aEntomologia 650 $aNomuraea Rileyi 700 1 $aBINNECK, E. 700 1 $aMARIN, S. R. R. 773 $tIn: ANNUAL MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 41.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS, 9., 2008, Warwick. Abstracts... Warwick: Society Invertebrate Pathology, 2008
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Embrapa Soja (CNPSO) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Roraima. Para informações adicionais entre em contato com cpafrr.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Roraima. |
Data corrente: |
11/03/2010 |
Data da última atualização: |
02/05/2012 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
TONINI, H.; ARCO-VERDE, M. |
Afiliação: |
Hélio Tonini, CPAF-RR; Marcelo Francia Arco-Verde, CPAF-RR. |
Título: |
Desempenho Silvicultural e econômico de clones de eucalipto na Amazônia setentrional. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: CONGRESO FORESTAL MUNDIAL, 13., 2009, Buenos Aires. Desarrollo forestal, equilibrio vital. Buenos Aires: República Argentina: FAO, 2009. |
Descrição Física: |
In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. |
Idioma: |
Português |
Thesagro: |
Silvicultura. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00630naa a2200145 a 4500 001 1692687 005 2012-05-02 008 2009 bl --- 0-- u #d 100 1 $aTONINI, H. 245 $aDesempenho Silvicultural e econômico de clones de eucalipto na Amazônia setentrional. 260 $c2009 300 $cIn: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 650 $aSilvicultura 700 1 $aARCO-VERDE, M. 773 $tIn: CONGRESO FORESTAL MUNDIAL, 13., 2009, Buenos Aires. Desarrollo forestal, equilibrio vital. Buenos Aires: República Argentina: FAO, 2009.
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